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Reaction Mechanisms of Pectinolytic enzymes⁽¹⁾:

Primary Structure of Pectin⁽¹⁾:

Pectolytic Enzymes Degrade Pectin Molecule:

Protopectinases⁽¹⁾:

They degrade the insoluble protopectin and give rise to highly polymerized soluble pectin. The enzyme that catalyzes the solubilization of protopectin was originally named protopectinase by Briton et al. Pectinosinase is also synonymous with protopectinase (PPase). Protopectinase catalyzes the following reaction:

                                                                  Protopectinase 

                          Protopectin (Insoluble) + H₂0           à             Pectin (soluble)

Esterases⁽¹⁾ 

                                                        Pectinesterase

                                              Pectin + H2O               à                 Pectate + CH3OH

                                                                            PECTATE STRUCTURE

The Image is collected from the following website:

 http://www.genome.ad.jp/dbget-bin/www_bget?rn+R02361+-s

Reaction Mechanisms of Depolymerizing enzymes⁽¹⁾                                                                                    

             Reaction mechanism from the above table:                           

                                                                              Action Mechanism